Journal: Research
Article Title: NRF2 Deficiency Disrupts Mitochondrial Homeostasis via NDUFS7 in Trabecular Meshwork
doi: 10.34133/research.1203
Figure Lengend Snippet: NRF2 activation in response to oxidative stress in the trabecular meshwork (TM). (A) Volcano plot of differentially expressed genes (DEGs) in human TM cells with or without tert-butyl hydroperoxide (tBHP) treatment. (B) Gene set enrichment analysis (GSEA) of human TM cells with or without tBHP treatment. (C) mRNA expression of NRF2, GPX4, GSR, SLC7A11, HMOX1, GCLM, NQO1, and GCLC in human TM cells with or without tBHP exposure. * P < 0.05, *** P < 0.001. (D) Protein expression of NRF2, GPX4, GSR, SLC7A11, HMOX1, GCLM, NQO1, and GCLC in human TM cells with or without tBHP exposure. (E) Immunofluorescence staining of NRF2 (red) and the TM marker α-SMA (green) in human corneoscleral rings, rhesus monkey eyes, and mouse eyes. Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue).
Article Snippet: Antibodies used were listed as follows: NRF2 (12721, CST), NDUFS7 (PA5-106367, Thermo Fisher Scientific), GSR (SC-133245, Santa Cruz Biotechnology), SLC7A11 (ab307601, Abcam), HMOX1 (10701-1-AP, Proteintech), GCLM (14241-1-AP, Proteintech), NQO1 (11451-1-AP, Proteintech), GCLC (12601-1-AP, Proteintech), GPX4 (67763-1-Ig, Proteintech), GAPDH (SC-137179, Santa Cruz Biotechnology), and β-actin (SC-47778, Santa Cruz Biotechnology).
Techniques: Activation Assay, Expressing, Immunofluorescence, Staining, Marker